Our Portfolio involves isolation and characterization of DNA or RNA for use in applications such as genotyping, gene expression analysis, epigenetic analysis, microbiome studies and more.
Experimental methods fundamental to nucleic acid analysis include nucleic acid extraction and clean up, DNA/RNA quantification, PCR, molecular cloning and sequencing.
Promega’s genomic DNA extraction offerings are engineered to isolate superior quality DNA from a diverse array of sources, such as blood, cells, tissues, plants, microbes, and more. You’ll find DNA extraction kits suited for all sample processing needs, from easy-to-use manual systems to benchtop automation, right up to tailorable chemistries for DNA isolation using robotic liquid handlers:
The quality and integrity of extracted RNA are critical for its effective utilization in methods like RT-PCR, RT-qPCR, and next-generation sequencing. Promega’s RNA purification products ensure the extraction of high-grade total RNA, mRNA or miRNA from a wide variety of sample types, such as blood, cells, tissues, FFPE tissue, plants, bacteria and viruses. Their product range caters to all sample throughput needs, offering solutions for manual purification to high-throughput automated systems.
For manual purification of total RNA or miRNA, whether in a single tube or 96-well-plate, Promega offers a selection of kits through their ReliaPrep™, and SV Systems. The PolyATtract® Systems are ideal for isolating mRNA from total RNA or crude lysates.
For those requiring benchtop-scale automated RNA purification from 1–48 samples, the Maxwell® RSC and Maxwell® 48 instruments and kits are equipped with convenient cartridges for specific sample types.
For high-throughput automated RNA extraction on liquid handlers, Promega provides SV 96 (silica membrane-based) and MagneSil® (magnetic-based purification) products.
We provide Miniprep, midiprep and maxiprep kit formats for plasmid DNA isolation from transformed bacteria, categorized by scale, enabling you to easily select the optimal plasmid purification kit for your requirements. These kits employ various processing alternatives, such as spin-based, magnetic-based, and vacuum-based techniques. No matter the processing approach, anticipate high yields and superior-quality plasmid DNA suitable for your subsequent applications.
Miniprep– from up to 10ml of bacterial cell culture.
Midiprep– from up to 250ml of bacterial cell culture.
Maxiprep– from up to 1000ml of bacterial cell culture.
PCR products and DNA resulting from restriction digests often require purification to eliminate nucleotides, primers, and other small molecules that might hinder subsequent applications. Promega offers versatile solutions for isolating and concentrating DNA or RNA fragments from PCR, gels, or for enhancing the concentration of DNA or RNA from weak solutions.
our best seller is Wizard® SV Gel and PCR Clean-Up System
A9281 | A9281 | A9285
Our best sellers:
Quantus™ Fluorometer E6150
GloMax® Microplate Reader GM3000 | GM3500 | GM3510
QuantiFluor® dsDNA/ssDNA/RNA System E2670/1 | E4870/1 | E3310 | E3190
Cloning a particular DNA fragment into a vector like a plasmid is a crucial step that facilitates further analysis of that sequence. Vectors from the pGEM® series offer several cloning sites suitable for fragments produced by specific restriction enzyme cuts. Fragments generated by PCR and similar TA cloning techniques can be seamlessly integrated into pGEM® T-vectors. For various subsequent applications, such as protein expression, the Flexi® Cloning System offers a convenient way to move fragments between different vectors.
Next, for typical cloning procedures you need competent cells which come in two variations: high efficiency, exceeding 108cfu/μg, suitable for cloning (like JM109, HB101), and a subcloning efficiency surpassing 107cfu/μg (such as HB101). An added benefit of JM109 cells is the blue/white screening capability to identify recombinants.
When aiming for protein expression, one can choose between JM109(DE3), BL21(DE3), or KRX competent cells. The Single Step (KRX) Competent Cells are tailored for effective transformation and precise protein expression control. These cells merge the top features of both processes, offering an optimized strain to assess protein expression in E. coli.
Our best sellers:
JM109 Competent Cells – L2005 | L2001 | L1001
pGEM®-T Easy Vector Systems – A1360
For all your PCR needs, from endpoint PCR to quantitative real-time PCR and RT-PCR, we provide an extensive range of top-notch products. Both endpoint and real-time PCR options utilize GoTaq® (Taq) DNA polymerase, available in handy master mixes, kits, and adaptable enzyme mixes for standard PCR, hot-start PCR, long-range PCR, qPCR, and RT-qPCR.
Our RT-PCR and real-time RT-qPCR selections feature both GoTaq® DNA polymerase and GoScript™ reverse transcriptase. RT master mixes include Promega renowned RNase inhibitor (also sold seperately).
Our kits ensure consistent and efficient reverse transcription and PCR, even with challenging templates or in the presence of PCR inhibitors.
In next-gen sequencing processes, we extract nucleic acid from a specimen, break it down, organize it into platform-specific libraries, then amplify and sequence it. Every phase in this process is vital, no matter the sample or platform.
Promega offers products like ProNex® NGS Quantitation and Size Selective DNA Purification Systems for pre-sequencing library prep, as well as tools for nucleic acid extraction and measurement. For Sanger sequencing tasks like verifying NGS base readings and confirming genome edits in altered cultures, we provide the Spectrum- a Compact benchtop Capillary Electrophoresis System and ProDye™ Terminator Sequencing System for fluorescent Sanger sequencing applications.
Promega provides a range of products designed to facilitate your studies in epigenetics. For DNA methylation studies, their offerings include assays for assessing methylation activity, kits for DNA purification, enzymes and mixes for PCR, restriction enzymes sensitive to methylation, and essential transfection reagents. Luciferase reporter assays are available for the investigation of various enzymes like HDAC, SIRT, and methyltransferases, as well as protease activities. Additionally, we can provide kits for purifying epigenetic proteins and identifying their interaction partners, which allows for quick and thorough analysis of histone modifications and protein associations.