Customized authentication services for mouse and human cell lines
STR loci are highly informative markers in the human genome, essential for verifying the quality and integrity of human cell lines in research. It’s established that at least thirteen core STR markers are necessary for accurate identification of human cell lines. The recommended markers include D5S818, D13S317, D7S820, D16S539, vWA, TH01, TPOX, CSF1PO, D8S1179, D3S1358, D18S51, D21S11, and FGA. These markers assist researchers in demonstrating the relatedness between cell lines and uniquely identifying human cells. Utilizing these 13 core loci allows for a discrimination rate of over 5.02 x 10^16 in distinguishing unrelated individuals.
ATCC conducts human STR profiling by amplifying multiple STR loci and the amelogenin gene (for gender identification) using Promega’s PowerPlex® 18D System. The amplified DNA fragments (amplicons) are separated through capillary electrophoresis and analyzed with GeneMapper® ID-X Software from Thermo Fisher Scientific. In the resulting electropherogram, each peak signifies an allele, which is scored alphanumerically and recorded in a database.
The ATCC Standards Development Organization released the ANSI/ATCC ASN-0002-2022 consensus standard, titled “Authentication of Human Cell Lines: Standardization of Short Tandem Repeat (STR) Profiling – Revised 2022”. This standard provides the research community with a globally recognized method for human cell line identity verification and offers guidance on interpreting STR profiling results.