We deliver relevant in vitro models and research tools, such as authenticated and characterized primary cells as well as immortalized, stem, and continuous cell lines. Disease and cellular pathway-specific cell line panels with genomic metadata and assay ready genomic DNA are also available.
Cell Culture
Cell Culture Maintenance
The ClinoStar technology is designed for culture of spheroids and organoids with minimal shear stress, enabling researchers to produce in vitro models that reproduce in vivo performance. This leads to more precise and consistent research and experiments, eliminating the need for animal testing.
Chemometec’s NucleoCounter® series of automatic cell counters and image analyzers stands out as the market’s most precise instruments. They offer simplicity in operation, and their automated nature ensures safe handling of samples.
Human Primary Cells
Primary cell cultures closely resemble the cells’ natural physiological state in the body, providing highly relevant biological data. They are directly isolated from organ tissues and cultivated in vitro. Therefore, primary cells, when maintained with suitable culture media and supplements, offer a more accurate and sophisticated model for mimicking specific tissue cell types compared to immortalized cell lines.
We offer:
- Large collection of primary cells from ATCC or Promocell
- Primary cells from patients and healthy donors from numerous tissue types
- Donor selection and HLA profiling available
Continuous Human and Animal Cell lines
ATCC maintains an extensive collection of cell lines, encompassing both diseased and healthy cells from a variety of human tissues. ATCC implements stringent quality control measures in its manufacturing processes, complemented by validated cell authentication techniques to verify the identity of all human cell lines.
Animal cells are crucial to biomedical research and bioindustrial applications, including producing recombinant proteins, generating viruses, detecting pathogens, and conducting toxicity screenings.
Choose from an extensive array of ATCC animal cell lines, encompassing over 150 different species, to support diverse research needs.
Rely on the foremost authority in cell authentication: ATCC provides researchers with a comprehensive suite of top-quality cell authentication testing services
hTERT Immortalized Cells
Human telomerase reverse transcriptase (hTERT)–immortalized primary cells mark a significant advancement in cell biology by melding the in vivo characteristics of primary cells with the in vitro longevity typically seen in continuous cell lines. ATCC has genetically modified human primary cells to possess the growth characteristics of continuous cell lines, including extended proliferative capacity, while preserving the physiological attributes of primary cells. This innovation bridges the gap between traditional primary cells and continuous cell lines, offering researchers a more robust and reliable cell model for various studies.
3D Cultures
3D cancer organoid cell models
ATCC, in collaboration with the Human Cancer Models Initiative (HCMI), offers scientists a comprehensive range of 2-D and 3-D patient-derived in vitro cancer models. This includes organoids and conditionally reprogrammed cells (CRCs), featuring a wide spectrum of both common and rare, less studied cancer types from various tissues. These models are all derived from human patients, and represent diverse genetic backgrounds. Detailed sequence data and patient clinical information are available for each model. Comprehensive and user-friendly culturing protocols specific to each model are also provided.
ATCC has been chosen as the exclusive distributor for the HCMI models. The institutions responsible for generating these models deposit them at ATCC, where they undergo authentication, expansion, preservation, and are subsequently made accessible for worldwide distribution.
Media supplements for 3D organoid or tumorsphere cultures.
ATCC has innovated Organoid Growth Kits. These kits consist of single-use supplements designed to simplify media preparation. They encompass the most expensive and complex supplements and reagents, thereby reducing the time and effort needed for media preparation and enhancing the likelihood of successful organoid growth.
Promocell also offers some unique Serum free solutions for 3D cultures of cancer and primary cells
The shift towards 3D culture methods like spheroids and organoids is becoming increasingly popular among researchers for studying cellular biology. 3D cell culture more accurately simulates the tissue or tumor environment compared to 2D cultures, as it allows for more natural interactions with a matrix and enables cells to secrete their own matrix. This approach also facilitates more authentic cell-to-cell contacts and creates a gradient in oxygen and nutrient availability, closely resembling natural conditions.
3D cell cultures often show significant differences in cellular responses when compared to traditional monolayer cultures. Analyzing cells in 3D involves examining variations in cell health, metabolism, and expression. These phenotypic changes are then correlated with the cell’s genotype or genetic alterations associated with disease states. We provide a comprehensive range of assays and tools tailored to each stage of your workflow, from assessing cell health and metabolic shifts to gene expression and analysis.
Reporter Cell lines
ATCC has integrated expression vectors carrying fluorescence, luciferase, or β-galactosidase reporter genes into numerous clinically important cell lines to reduce the time and costs involved in developing reporter-labeled cells.
The resulting cell products have undergone extensive evaluation for:
– Expression of the reporter
– Stability of the vector
– Suitability with detection methods
– Rate of cellular growth
– Cell shape and structure
Promega is broadening its array of HiBiT, NanoLuc®, and HaloTag® CRISPR knock-in cell pools and clones of widely studied drug targets. See our collection of CRISPR-engineered cell lines, featuring a bioluminescent/fluorescent marker linked to the desired target, offering a measurable approach to investigate natural protein levels. These cell lines enable live-cell assays for real-time observation of protein behavior.
Stem Cells
Stem cells possess the unique ability to develop into various cell types in the body. They share three key characteristics: the capacity for long-term self-renewal, being unspecialized, and the ability to differentiate into specialized cells.
Stem cells hold potential in regenerative medicine as a source for replacing cells and tissues to treat a variety of conditions.
See our collection from various vendors of reliable stem cell resources , offering a diverse range of cultures, including mouse embryonic, human mesenchymal, neural progenitor, and induced pluripotent stem cells alongside culture solutions to support the growth and maintenance of these cells or support differentiation and reprogramming
Human Serum
Human Male AB Plasma-Derived Serum
The Human Male AB Serum we offer is an exclusive product produced using a cGMP process, utilizing either human or bovine thrombin.
Male AB Off the Clot serum
After collection, the whole blood is allowed to naturally coagulate. Once the clot has formed, the material undergoes centrifugation to separate the clot and obtain the serum, which is subsequently subjected to viral testing.
Heat inactivation and/or gamma iradiation available
Cell Culture Media
Cell culture involves a variety of methods used to sustain and nurture cells outside of their native tissue setting, in an environment where survival conditions are carefully managed.
We ensure that all our products undergo thorough quality assurance checks, including tests for pH, osmolality, composition, uniformity, glucose and sodium content, endotoxin presence, and biological efficacy.
Proprietary media formulations for primary human or animal cell cultures from ATCC or Promocell
CE/IVD media for clinical cytogenetics
LymphoGrow Medium – ready-to-use solution,for growing primary human blood lymphocytes, vital for karyotyping and various other postnatal cytogenetic diagnostic methods.
Thanks to its finely tuned concentration of phytohaemagglutinin (PHA), LymphoGrow Medium enhances the mitotic index, ensuring superior chromosome morphology and banding patterns for chromosome analysis.
This product contains penicillin/streptomycin and PHA.
AmnioGrow Plus Medium (AGM) – ready to use medium, for the culture of primary human amniotic cells and chorionic villi, intended for use in human karyotyping and various prenatal cytogenetic diagnostics.
This medium facilitates highly effective cell growth and adherence, leading to early chromosome analysis and the production of high-quality karyograms.
Contains validated Fetal Bovine Serum (FBS) and gentamycin.
MarrowGrow Medium – ready-to-use medium for cultivating hematopoietic cells derived from bone marrow and leukemic blood samples, primarily utilized for karyotyping and various cytogenetic diagnostic processes, designed to enhance cell growth and adherence, facilitating chromosome analysis.
This product contains validated FBS and gentamycin.
Prenaplus Medium – formulated for the cultivation of cells from amniotic fluid, chorionic tissue, and abortive material, specifically designed for use with human samples, complying with the CE/IVD.
The medium contains validated FBS and a range of antibiotics.
Serum & plasma products (from various species including many modifications of FBS)
Explore our selection of FBS, animal sera, BSA, and human-derived products.
Every production batch undergoes strict control, from the initial serum collection through processing, manufacturing, and final packaging. Each order is accompanied by a Certificate of Analysis, certifications of origin and complete traceability for all sera
Cancer Cell culture
Cancer cells found in tumors or established lines are diverse, including:
- Slow-growing tumor-initiating cancer stem cells
- Rapidly proliferating aggressive cancer cells
- Tumor-associated cancer cells
- Immune and other cell types based on your research design
Promocell has devised a set of tools aimed at isolating, cultivating, and co-culturing these various cell types long-term.
The toolbox comprises three media options that all support the maintenance of cancer stem cells (CSCs) that are critical due to their role in tumor formation, growth, and spread. All three media types are synthetic, devoid of animal components, and xeno-free. They are designed to support growth and long-term cultivation in 2D or 3D environments and are compatible with most established cell lines and newly developed lines from various cancer types.