Why Choose Vectron?
Whether you are a pharmaceutical entity grappling with inclusion body formation in pre-clinical drug development or an enzyme production company eager to elevate the titers of your latest product, Vectron perceives every protein challenge as a puzzle awaiting resolution.
Tailored Solutions for Unique Challenges
At the heart of Vectron’s advanced technologies is the bacterial promoter Pm and its corresponding transcription regulator gene, xylS. Notably, Pm stands out as an inducible promoter with characteristics that set it apart:
- Tight control when uninduced and robust expression during maximal induction.
- Activation using affordable, non-toxic benzoic acids, which are harmless to both bacteria and humans.
- Avoidance of the typical “all-or-nothing” effect seen with other inducers like IPTG.
- Proven record-high protein titers (up to 60-90g/L) with high quality and purity
Through meticulous engineering, Vectron has pioneered expression cassettes for E. Coli that are characterized with:
- Minimal metabolic burden.
- High vector stability without the need for antibiotic selection.
- Potential for boosting vector copy numbers for heightened titers.
Achieving Peak Solubility in E. coli
- Finely-tuned Expression: The Pm/xylS system’s precision prevents overloading the folding machinery, ensuring optimal protein solubility.
- Efficient translocation to the periplasm: With a rich library of signal peptides, Vectron ensures efficient disulfide bond formation for efficient translocation to the periplasm.
- Improved Solubility: Vectron harnesses fusion proteins, chaperones, and cultivation tweaks to optimize solubility.
Attaining high soluble protein levels in E. coli, especially human proteins, demands a multi-pronged strategy. Vectron’s expertise encompasses:
Pushing Boundaries with Directed Evolution
Beyond their expansive library of pre-identified expression cassettes, Vectron is pioneering a revolutionary expression vector design, whereby billions of randomized expression vectors are being generated and screened to incrementally improve the vectors’ sequences and identify unique and optimal expression vectors for each protein.
The result? Expression vectors that evolve to outperform any pre-established limitations.
An Upstream Solution to Downstream Challenges
Industrial protein production in E. coli often faces hurdles like protein degradation, misfolding, and increased downstream costs due to protein accumulation in inclusion bodies. To overcome these issues Vectron developed a solution designed to transport proteins outside the cell using genetically modified E. coli strains that synthesize flagella, serving as conduits for directed protein secretion. Particularly beneficial for hard-to-express or toxic proteins, an additional advantage being significant cost reduction by bypassing difficult downstream purification from inclusion bodies.
Vectron Biosolutions is not just a provider of solutions but an innovator reshaping the landscape of protein synthesis. Partner with Vectron and IMBH,together, we’ll unlock the future of protein production.
All information is based on the manufacturer’s website.