MEM (Minimum Essential Medium), with Earle’s Salts, with Non-Essential Amino Acids (NEAA), is a versatile cell culture medium that supports the growth and maintenance of various mammalian cells. The formulation of MEM is based on Eagle’s medium, providing essential nutrients, salts, and buffering agents to promote optimal cell growth. The addition of NEAA further enhances cell viability and proliferation by providing cells with additional nutrients that they do not synthesize.
Applications:
- Culturing of mammalian cell lines
- Cell biology research
- Supporting the growth of adherent and suspension cultures
Benefits:
- Contains Earle’s salts for maintaining physiological pH in CO2 incubators
- NEAA added to improve cell viability and growth
- Formulated without L-Glutamine to allow for specific supplementation needs
Key Components:
- Earle’s Salts
- Non-Essential Amino Acids (NEAA)
- Balanced electrolytes and buffering agents
Usage Instructions: Supplement with L-Glutamine and serum or other additives as needed for specific cell lines before use. Use under sterile conditions to maintain aseptic environments.
Storage Conditions: Store at 2-8°C