AnyGenes is a leading provider of molecular biology reagents and assays, specializing in gene expression, cell signaling, and biomarkers profiling
In the fast-evolving field of biotechnology, innovations often take the potlight. These innovations revolutionize our understanding of complex biological systems and diseases. Moreover, researchers have embraced the use of quantitative Polymerase Chain Reaction (qPCR) arrays as a key innovation. This technique is a cornerstone in molecular biology and genomics research
This article delves into the specifics of qPCR arrays, their significance in research, and how they are implemented in studies, with a focus on AnyGenes‘ contributions to this area.
Introduction to qPCR Arrays
qPCR arrays are the pinnacle tools for parallel quantitative analysis. They focus on gene expression signatures from a specific panel of genes. Researchers use these arrays for mRNA and microRNA expression profiling. They are available in 96-well and 384-well plate formats. qPCR arrays use SYBR Green I or comparable DNA-binding dyes for detection. They also incorporate optimized probe-based primer sets, such as TaqMan or LNA probes. This versatility allows comprehensive screening across entire panels of signaling pathways or expressed microRNA. Moreover, qPCR arrays can be customized to include gene panels tailored to specific research needs. This customization enhances the understanding of cell signaling pathways and biomarker profiling.
Additionally, other uses of qPCR arrays, particularly by Anygenes, include the analysis of microbiota presence in various species. They are also used for the analysis of long non-coding RNAs. Extra tools enable RNA analysis from challenging, low-availability sources. Options include cDNA pre-amplification using the speAmp kit, which allows the analysis of 368 genes from as little as 5ng of RNA. Another option is direct lysis for isolating cDNA directly without RNA extraction, even from ultra-low sample sizes of just 10-100 cells
Why qPCR Arrays?
This technique is the preferred choice for several reasons:
- Pathway Focused: They enable the profiling of selected genes’ expression relevant to specific pathways or disease states. This profiling provides insights into complex biological processes.
- Simple and Accurate: The simplicity of the real-time PCR method, combined with the advantages of qRT-PCR technology, offers high sensitivity. It also provides a broad dynamic quantification range.
- Quantitative and Reproducible: When researchers apply qPCR arrays correctly, they become fully quantitative and exhibit high reproducibility. This reliability is crucial for consistent research findings.
- High Throughput: qPCR arrays are designed for high throughput applications. They make expression profiling accessible to laboratories equipped with block-based real-time PCR instruments.
Performance and Applications
qPCR arrays exhibit exceptional performance characteristics:
- Sensitivity: Capable of detecting as little as 1 ng per reaction setup.
- Reproducibility: With replicate correlation coefficients greater than 0.99, qPCR arrays facilitate reliable comparison of experimental samples across different runs and plates.
- Specificity:The specificity of the system, using validated primer pairs designed from two different exons, ensures the generation of a single product of predicted size from every reaction, minimizing the occurrence of secondary products.
How qPCR Arrays Work
The workflow of qPCR arrays is straightforward. First, mix the cDNA template with a ready-to-use PCR master mix. Then, aliquot equal volumes into each well of the plate and run the real-time PCR cycling program. Additionally, the well-designed plate layout includes multiple controls for genomic DNA contamination, RNA quality, and PCR performance. Researchers also include multiple reference genes to ensure reliable results. Furthermore, data analysis is streamlined and often conducted via an Excel-based template. This process utilizes the ΔΔCt method for normalization and expression profiling.
How do you quantify gene expression using qPCR?
The amount of gene expression is quantified by comparing the cycle threshold (Ct) values of target genes to those of reference genes, allowing for accurate and sensitive measurement of gene expression levels across samples with different treatments or physiological phenotypes.
AnyGenes’ Role in Advancing qPCR Array Technology
AnyGenes excels in the biotech industry by providing a wide array of qPCR array products and services, significantly contributing to research in diverse areas. Their precision tools have advanced the understanding of disease pathogenesis and facilitated the development of new therapeutic approaches. Through collaborations with academic and pharmaceutical research entities, AnyGenes continues to be at the forefront of molecular biology and genomics innovation.
In conclusion, qPCR arrays represent a crucial tool in the molecular biologist’s arsenal, offering unparalleled precision and reliability for gene expression analysis. AnyGenes’ commitment to providing cutting-edge tools and services focused on molecular biology and qPCR arrays underscores its vital role in propelling scientific discovery and innovation in biotechnology.
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