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    Decoding the Code: AnyGenes’ Innovative Approach to Biomarker Profiling
    26 Feb 2024

    Decoding the Code: AnyGenes’ Innovative Approach to Biomarker Profiling

    Revolutionizing Molecular Biology and Genomics: AnyGenes at the Forefront of Advancing Research and Innovation in Biotechnology. Pioneering New Frontiers in Understanding Complex Biological Systems and Diseases, Through Cutting-Edge Tools and Collaborative Science.

    In the fast-evolving field of biotechnology, the spotlight often shines on innovations that revolutionize our understanding of complex biological systems and diseases. One such innovation is the use of quantitative Polymerase Chain Reaction (qPCR) arrays, a cornerstone technique in molecular biology and genomics research.

    This article delves into the specifics of qPCR arrays, their significance in research, and how they are implemented in studies, with a focus on AnyGenes’ contributions to this area.

    Introduction to qPCR Arrays

    qPCR arrays stand as the pinnacle of tools for the parallel quantitative analysis of gene expression signatures from a focused panel of genes. These arrays are pivotal in mRNA and microRNA expression profiling, available in 96-well and 384-well plate formats. The detection chemistry of qPCR arrays employs SYBR Green I or comparable DNA-binding dyes, alongside optimized probe-based primer sets, including TaqMan or LNA probes. This versatility allows for comprehensive screening across entire panels of signaling pathways or expressed microRNA. Moreover, qPCR arrays offer customization to include gene panels tailored to specific research needs, enhancing the understanding of cell signaling pathways and biomarker profiling. Other uses of qPCR arrays and specifically those by Anygenes, include analysis of microbiota presence in a variety of species and applications and analysis of long non-coding RNAs as well.
    Extra tools allow analysis of RNA from challenging, low availability sources with options like cDNA pre-amplification using the speAmp kit which enables analysis of expression of 368 genes from as little as 5ng of RNA or the direct lysis option for isolation of cDNA directly without RNA extraction from ultra-low sample sizes of 10-100 cells only.

    Why qPCR Arrays?

    qPCR arrays are the method of choice for several reasons:

    • Pathway Focused: They enable the profiling of selected genes’ expression relevant to specific pathways or disease states, providing insights into complex biological processes.
    • Simple and Accurate: The simplicity of the real-time PCR method combined with the advantages of qRT-PCR technology offers high sensitivity and a broad dynamic quantification range.
    • Quantitative and Reproducible: When applied correctly, qPCR arrays are fully quantitative, exhibiting high reproducibility, which is crucial for ensuring consistent research findings.
    • High Throughput: Designed for high throughput applications, qPCR arrays make expression profiling accessible to laboratories equipped with block-based real-time PCR instruments.

    Performance and Applications

    qPCR arrays exhibit exceptional performance characteristics:

    • Sensitivity: Capable of detecting as little as 1 ng per reaction setup.
    • Reproducibility: With replicate correlation coefficients greater than 0.99, qPCR arrays facilitate reliable comparison of experimental samples across different runs and plates.
    • Specificity:The specificity of the system, using validated primer pairs designed from two different exons, ensures the generation of a single product of predicted size from every reaction, minimizing the occurrence of secondary products.

    How qPCR Arrays Work

    The workflow of qPCR arrays is straightforward: mix the cDNA template with a ready-to-use PCR master mix, aliquot equal volumes into each well of the plate, and run the real-time PCR cycling program. The well-designed plate layout includes multiple controls for genomic DNA contamination, RNA quality, and PCR performance, including multiple reference genes, ensuring the reliability of the results. Data analysis is streamlined, often conducted via an Excel-based template, utilizing the ΔΔCt method for normalization and expression profiling.

    How do you quantify gene expression using qPCR?

    The amount of gene expression is quantified by comparing the cycle threshold (Ct) values of target genes to those of reference genes, allowing for accurate and sensitive measurement of gene expression levels across samples with different treatments or physiological phenotypes.

    AnyGenes’ Role in Advancing qPCR Array Technology

    AnyGenes excels in the biotech industry by providing a wide array of qPCR array products and services, significantly contributing to research in diverse areas. Their precision tools have advanced the understanding of disease pathogenesis and facilitated the development of new therapeutic approaches. Through collaborations with academic and pharmaceutical research entities, AnyGenes continues to be at the forefront of molecular biology and genomics innovation.

    In conclusion, qPCR arrays represent a crucial tool in the molecular biologist’s arsenal, offering unparalleled precision and reliability for gene expression analysis. AnyGenes’ commitment to providing cutting-edge tools and services focused on molecular biology and qPCR arrays underscores its vital role in propelling scientific discovery and innovation in biotechnology.

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    Beit Haemek, Israel 2511500

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      IMBH - Import and Marketing Beit HaEmek, Israel.
      Distributor and supplier of ATCC, Promega, Promocell, Chemometec, Biowest, LSG, Reprocell, AnyGenes, ABClonal, CelVivo, Softgenetics, Filtrox, Quatroflow, Amazon Filters, Industrial Filters and Vectron.
      Biotechnology products for molecular biology, cell analysis, cell culture, genetic identification, microbiology, protein analysis, advanced filtration for the food and beverage market and unique filtration solutions for the pharmaceutical and biotechnology market.

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